¸ŁŔűĽ§

18 December 2023

Scientists, including Linda Bojmar as first author, have introduced a comprehensive protocol for the cross-platform characterization of extracellular vesicles and particles (EVPs) in both humans and mice.

The protocol aims to provide a standardized approach for analyzing EVPs, crucial for unraveling their functions and exploring biomarker potential.

Key Highlights of the Protocol

Cross-Validation Across Platforms: The protocol emphasizes the cross-validation of isolated EVPs across different platforms, ensuring robust and consistent results.

Multifaceted Analysis: Researchers can now employ various techniques for EVP analysis, including nanoparticle tracking analysis and transmission electron microscopy, allowing for the examination of EVP size, number, and morphology.

Protein Cargo Identification: The protocol introduces a novel approach to EVP protein cargo identification using liquid chromatography-tandem mass spectrometry (LC-MS/MS) coupled with bioinformatic analysis.

Validation Techniques: To verify the presence of specific EVP proteins, the protocol advocates for single-particle analysis, western blot analysis, and ExoELISA, providing a comprehensive validation framework.

The significance of this protocol lies in its ability to facilitate EVP cross-validation across different platforms, enhancing the reliability of research outcomes. By offering a standardized methodology, scientists can now delve deeper into the intricate world of extracellular vesicles and particles, paving the way for breakthroughs in understanding their roles and potential as biomarkers.

This protocol is expected to catalyze advancements in the study of EVPs, opening new avenues for therapeutic development and diagnostic applications.

Contact

Latest news from LiU

A view of a space shuttle flying over the audience in the dome.

Anyone can land on the moon with a new immersive film

The film Once Upon the Moon allows the audience to experience the moon landings as if they were there themselves. Authentic footage, astronauts’ own stories and the latest visualisation technology make this possible.

En kopp som stĂĄr pĂĄ ett bord.

LEAD appointed as Swedish accelerator for NATO DIANA

LEAD, an innovation incubator based in Östergötland, has been appointed Swedish accelerator for NATO DIANA – NATO’s innovation programme for the development of new technologies with both civilian and military applications.

A woman standing in front of a laptop computer.

LiU educates elected representatives on AI and societal impact

LiU is launching a learning platform that gathers research-based knowledge about the impact of artificial intelligence and digitalisation on our democracy. The aim is to equip politicians for a new reality where AI is an integral part of society.